~/.homebrew/brew.env: How-To specify two different HOMEBREW_CASK_OPTS?

This is my brew.env. cat ~/.homebrew/brew.env HOMEBREW_CASK_OPTS="--no-quarantine" HOMEBREW_CASK_OPTS="--appdir=~/Applications" HOMEBREW_CLEANUP_MAX_AGE_DAYS=2 HOMEBREW_NO_ANALYTICS=1 The second line HOMEBREW_CASK_OPTS="--appdir=~/Applications" is working, but first line HOMEBREW_CASK_OPTS="--no-quarantine" is ignored. How to specify both env variables? I tried also HOMEBREW_CASK_OPTS="--no-quarantine --appdir=~/Applications" on the same line, but both variables did not work.