Filter duplicated based on values of another column

I have the following example of a dataframe.Where you see that elements of 3nd column could be duplicated.I want to keep the entry which has the highest value in column 5 Meaning that for **AGCCCGGGG** I want to keep the second entry which the 5th column has the value of 49. A00643:620:HFM7YDSX5:1:1124:7120:12352 ATCAGCCCGGGGCTTGGGCTAGGAC GGGTGTGTG 548476 0 Corynebacterium A00643:620:HFM7YDSX5:1:1150:15953:12524 CCTATCGTCGCTGGAATTCCCCGGG AGCCCGGGG 1458266 1 Bordetella A00643:620:HFM7YDSX5:1:1150:15628:12743 CCTATCGTCGCTGGAATTCCCCGGG AGCCCGGGG 1458266 49 Bordetella A00643:620:HFM7YDSX5:1:1450:4001:4507 GGCGATCGAAATGTCAAGCCCGGGG TCTTGTGGT 585529 0 Corynebacterium A00643:620:HFM7YDSX5:1:2124:8865:2472 ATCAGCCCGGGGCTTGGGCTAGGAC GGGTGTGTG 548476 0 Corynebacterium A00643:620:HFM7YDSX5:1:2476:4001:29496 ATTCACCCTATAGGAGCCCGGGGCA TGCCCCGGG 1458266 0 Bordetella